Latest Status: equipment for v0 purchased and in transit. Possibly lost in mail until 12/27. Will update.
I'm going to setup my own home lab for imaging mitochondria. I'm going to do it as cheaply and reproducibly as possible so anyone can copy the setup. The goal is to create the world's largest public domain database of mitochondria images. Follow me if interested!
This script (mitocounter.py) can be used to take an image of stained mitochondria and count them.
Example input:
Example output:
Are there cheap fluorescence microscopes?
Can we build our own fluorescence microscopes? We only need 1 range.
Likely going to need a scope with oil immersion
Beginners watch this (@micro_safari - microsafari.org)
Suppliers:
Rhodamine 123
Thermofischer Scientific - 25mg for $139.65
Janus Green B
methylene blue
Cheek (buccal) epithelial cells
Light microscopy of mitochondria at the nanoscale
Analysis of mitochondrial dynamics and functions using imaging approaches
Live cell microscopy of mitochondria-lysosome contact site formation and tethering dynamics
Mitochondrial Dynamics in the Regulation
of Nutrient Utilization and Energy Expenditure
Mitochondrial volume densities in the smokeless
tobacco-treated hamster cheek pouch epithelium
~1 micron in diameter
Symposium on Mitochondria at the School of Medicine, Westlake University
6th Annual Mitochondria Symposium
https://x.com/Mito_Therapy/status/1868212807965000114
Can we rig up a spectrophotometer approach? Do we need imaging, or can we get by with just dye + color reading?
$89.99Swift Microscope | $48.99Swift 1.3 Megapixel Digital Camera | TBDCustom Dye |
12/12/2024 - Funding secured! Thanks to the Warpcast community, we launched $MITO and raised over $2,000 to fund the PowerHouse Project.
12/12/2024 - Added mitocounter script.
12/12/2024 - Website launched
12/12/2024 - @Ayyy_Unit tweets name idea
12/12/2024 - Breck tweets idea
Just a place to write down names of folks providing help/advice.
Clone this site:
git clone https://powerhouse.breckyunits.com/powerhouse.breckyunits.com.git